Human glutamic-γ-semialdehyde dehydrogenase. Kinetic mechanism

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Succinic Semialdehyde Dehydrogenase

The enzyme succinic semialdehyde dehydrogenase from pig brain has been 2000-fold purified by a combination of DEAE-cellulose, hydroxyapatite, and AMPSepharose chromatography. This preparation has a molecular weight of 160,000 and a specific activity of 5.3 pmol/min*mg at 25°C. The inhibition of succinic semialdehyde dehydrogenase by carbonyl compounds, i.e. P-pyridoxal and o-phthalaldehyde was ...

متن کامل

Kinetic and equilibrium studies on crystalline 1-glutamic acid dehydrogenase.

The spectrophotometric procedure for determining the specific activity of the enzyme and the preparation of reagents employed in the assay have been described previously (3). DPiV-Commercial preparations of DPN (60 per cent) were used in routine assays. For critical experiments, commercial DPN was further purified by several adsorptions on Dowex 1 formate columns by the method of Kornberg and P...

متن کامل

Kinetic mechanism of chicken liver xanthine dehydrogenase.

The kinetic behaviour of chicken-liver xanthine dehydrogenase (xanthine/NAD+ oxidoreductase; EC 1.2.1.37) has been studied. Steady-state results, obtained from a wide range of concentrations of substrates and products, were fitted by rational functions of degree 1:1, 1:2, 2:2 and 3:3 with respect to substrates, and 0:1, 1:1, 0:2 and 1:2 with regard to products, using a non-linear regression pro...

متن کامل

Purification and characterization of methylmalonate-semialdehyde dehydrogenase from rat liver. Identity to malonate-semialdehyde dehydrogenase.

Methylmalonate semialdehyde dehydrogenase was purified from rat liver in order to define the distal portion of valine catabolism and related pathways in mammals. The purified enzyme is active with malonate semialdehyde and consumes both stereoisomers of methylmalonate semialdehyde, implicating a single semialdehyde dehydrogenase in the catabolism of valine, thymine, and compounds catabolized by...

متن کامل

Glutamic Acid Dehydrogenase and Glutamic-oxalacetic

As part of a larger project dealing with an in vestigation of amino acid and protein metabolism in leukemia, a study was undertaken to determine those enzymes concerned with amino acid anabo lism and catabolism as they occur in selected tis sues. Several review papers (@,4, 11, 1@)have ade quately summarized our present knowledge of the metabolism of white blood cells (WBC) in leuke mia. Howeve...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1989

ISSN: 0264-6021,1470-8728

DOI: 10.1042/bj2610935